Diffusion tensor imaging(DTI) and its application in visual development and amblyopia research / 眼科研究

Diffusion tensor imaging(DTI) is an efficient and noninvasive tool to investigate the characteristics and functions of white matter fiber tracts,and it can be used to study the relationship between the structure and function in the nervous system,the plasticity and damage repair of visual development,abnormalities of the white matters that relate to the visual pathways.Recently,the quantification of white matter fiber tracts from the lateral geniculate bodies to the occipital lobe visual cortex using DTI offers morphological information for the function and structure of the white matters during visual development.Some studies attempt to compare the DTI outcomes and tractography between amblyopic children and normal children so as to better display the variations of optic radiation in children with amblyopia,and to evaluate the relationship between structure and function of the white matters.DTI becomes an important supplementary research approach in these fields.This paper reviews the application of DTI in visual development and amblyopic eyes.

Effect of conditioned medium with concanavalin A-activated spleen cells supernatant on cultured rabbit corneal endothelial cells / 西安交通大学学报(医学版)

Objective To evaluate the effect of conditioned medium made up of ConA-activated spleen cells supernatant on cultured rabbit corneal endothelial cells (RCECs).Methods RCECs were primarily cultured in vitro by revealing descemet membrane and endothelium combining tissues masses.Culture medium was RPMI 1640 with 10% FBS and varied concentrations of conA-activated spleen cells supernatant.Experimental groups were divided by different concentrations of ConA-conditioned supernatant:5%,10%,15%,and 20%.In the control group only RPMI1640 and 10% FBS were used.Methyl thiazolyl tetrazolium colorimetry analysis was used to evaluate the proliferation of RCECs cellular population.The cultured cells were identified by immunohistochemical staining for neuronal specific enolase (NSE).Results The RCECs were cultured successfully.RCECs presented positive expression in cytoplasm with NSE immunohistochemical assay.There were significant differences in the experimental groups compared with the control group (P